Resources

Technical Videos

Watch short videos on techniques such as:

  • Fluorescent microscopy
  • Antibody conjugation
  • Invasion & migration assays

  • Cell culture & cell counting
  • ELISAs
  • Flow cytometry


  • 2D gel electrophoresis
  • Primer design
  • Western blot and more …


Protocols

Protocols are available for techniques including Immunohistochemistry, Western Blot, Sandwich ELISA, ELISA Pair, Fluorescent In Situ Hybridization, RNAi Knockdown, Immunoprecipitation, Kinase Assay, and more!

Plant Science Events Calendar
Sign-up your meeting, workshop, symposium or check what is coming!
Plant Science Events Calendar.jpg

How to Cite an Antibody in a Scientific Publication?
As more than half of the antibodies used in research cannot be properly identified, it is absolutely crucial to include information, which will allow antibody identification by researchers who want to repeat your experiments. Some journals provide a guideline for antibody citation, unfortunately most of them do not. This issue is most often completely overlooked. Find out how to cite your antibody here.

Posters on Photosynthesis
Updated photosynthetic posters developed by Professor Govindjee, Professor Lars Olof Björn and  Dr Dmitry Shevela,are available in format A0 to decorate your office or lab wall. Contact Sapphire Bioscience to order a complimentary print copy of this poster.
Antibodies_Inc_camelids.jpg
Alpaca-derived nanobodies as part of the antibody ‘toolbox’

Rabbit and mouse antibodies are mainstay reagents found in all biological research labs, but some of you have likely heard the buzz around camelid antibodies. Camelids include camels, llamas, alpacas, and other lesser known species, though llamas and alpacas are the most commonly used camelids for antibody development since they are easier to handle....

Read the Antibodies Incorporated Blog >


BioLamina Technical Videos

  • Benefits of the Biolaminin 521 cell culture system | Watch Video
  • Coating cultureware with Biolaminin substrates | Watch Video
  • Passaging and culture of Human PSC on Biolaminin 521 | Watch Video
  • HPSC transition to Biolaminin 521 | Watch Video
  • Watch as cells grow - Culture ES and IPS cells on Laminin | Watch Video

Technical Guides
  • STEM CELL CULTURE MATRICES - A practical guide | Download PDF

Technical Notes

Technical Tips for ELISA Assays (pdf)

Spike-and-recovery and linearity-of-dilution assays are two essential validation studies that assess the ability of the ELISA assay to measure the true (ie. accurate) amount of antigen in the sample.

Bioss is dedicated to helping you achieve exceptional results. Their top-notch scientific support team has worked hard to develop these protocols for all their applications. They hope these instructional aids will assist you in your research!


PROTOCOLS


NEWS/ARTICLES


Cayman Chemical offers various tools and resources to support their products and services and help make your research possible.


Technical Support: Contact Cayman Chemical Scientists for help with selecting a Cayman product or help with a purchased product

Analysis Tools: Analyse assay kit results with Microsoft Excel-based quantitation templates. 

Assay Kit Recommendation Guide: Compare assay kits based on sample type, target, and preferred detection method

Batch Specific Data and Documents: Download or request product inserts, QC sheets, certificates of analysis, and GC-MS data

Chemical Structure Database: Download the latest compound database in MDL SD file format 

ELISA (Competitive) Analysis Tools: Download Excel Workbooks for assays performed in triplicate (ELISATriple) or duplicate (ELISADouble)

Literature & Media: Download or request lab wall posters, application notes, brochures, scientific posters, and more

Molarity Calculator: Determine the mass, concentration, or volume required to make a solution.

Molar Mass Calculator Tool: Determine the Molar mass and Percent mass composition of a linear chemical formula.

Fluorescence Spectra Viewer: Use this interactive spectra viewer to compare excitation and emission wavelengths of 900+ fluorophores. Multiplex different fluorophores and design experiments to help you find the best products for your application.

Cayman News: Read the newest announcements, articles, and press releases from Cayman


Forensic Chemistry & Toxicology

Cayman Spectral Library: Search the GC-MS spectral database available in Agilent MSD ChemStation and NIST file formats 

GC-MS Drug Identification Search Tool: Search unknowns against formula weight, base peak, or 2nd base ion

Synthetic Cannabinoid Flipbook: Explore structural similarities to known Schedule 1 synthetic cannabinoids

SpectroLearn | An educational portal where you can find content that will cover all things spectral cytometry.

FSP™ Panel Design Video Series
The Cytek® FSP Panel Design Series covers a range of topics that will help you learn fundamentals of flow cytometry. This series will dive deep into Full Spectrum Profiling™ Technology (FSP) so that you can get the most out of your Cytek® Aurora, Northern Lights and Aurora CS systems.

Reference Controls Video

Webinars:


Blog Articles:

Reagent Selection Guide for Cell Proliferation/Cell Viability/Cytotoxicity


Antibody Search Tools
Enzo Life Sciences offers thousands of antibodies against peptides, whole or partial proteins, and modified peptides or proteins. Backed by thousands of peer-reviewed citations and expert technical support, you can count on Enzo antibodies to deliver reliable, consistent, accurate data.
  • Rigorously validated for WB, IP, ELISA, IHC, ICC, IF, and flow cytometry
  • Superb performance documented in thousands of peer-reviewed publications from 1986-present
  • Expanded utility provided by common fluorophore and enzyme conjugates
  • Convenient secondary antibodies and positive control lysates and proteins
  • Bulk production and custom conjugation/formulation services to meet your needs


Technical Videos | View All

  • Immunohistochemistry (IHC)
  • Immunofluorescence Assay (IFA)
  • Western Blot (WB) – Sample preparation, Electrophoresis


Protocols | View All

A collection of useful printable protocols and technical tips available for antibody applications:

  • Protocol - Western Blot
  • Protocol - ICC/IF
  • Protocol - IHC (paraffin)
  • Protocol - IHC (frozen)
  • Protocol - Western Blot
  • Protocol - ICC/IF
  • Protocol - IHC (paraffin)
  • Protocol - IHC (frozen)
  • Protocol - IHC (whole-mount)
  • Protocol - Immunoprecipitation
  • Protocol - ELISA (sandwich)
  • Protocol - ELISA (indirect)
  • Protocol - Flow Cytometry
  • Protocol - Dot Blot
  • Protocol - Blocking Peptide Competition
  • Protocol - Cytosolic and Membrane Fractionation

GeneTex Webinar Series | View All
  • Viral and Host Determinants of Arbovirus Viremia and Dissemination
  • The interface of the ovary and fallopian tube during ovarian cancer formation and spread
  • SARS-CoV-2 Omicron therapeutics: Getting to know the unknown and the road ahead
  • Blood plasma-derived extracellular vesicles: A minimally invasive tool for predicting response to cancer treatment
  • RAS Genes in Development and Cancer

Technical Protocols


List Labs Blog

  • Pertussis Toxin Application in Multiple Sclerosis Research - Read the article >
  • Cell Ablation Using Diphtheria Toxin (DT) is an Important Technique for Studying Regeneration in Living Animals - Read the article >

Histology Protocols
  • Preparation of fresh or frozen tissue samples for sectioning - View Protocol >
  • Formalin-fixation and paraffin-embedding of cells and tissues - View Protocol >

Immunohistochemistry (IHC)

  • IHC-plus™ Immunohistochemistry Protocol - View Protocol >
  • Immunohistochemistry protocol for formalin-fixed, paraffin-embedded tissues using Proteinase K based antigen retrieval - View Protocol >
  • Immunohistochemistry protocol for formalin-fixed, paraffin-embedded tissues using steam based antigen retrieval - View Protocol >
  • Immunohistochemistry protocol for double-labelling of formalin-fixed, paraffin-embedded tissues (for visualising two different primary antibodies (DAB and Vector Red chromagens) - View Protocol >

Mercodia Webinars - View the latest webinar recordings

Mercodia webinars connect you with experts in various scientific fields.  These special sessions are educational, informative and often provide you with answers to questions that may assist your group in making decisions now or in the future.  All of our webinars are recorded and posted below as an added resource for our valued customers.

  • Bariatric Surgery: Regulation of Gut and Islet Hormones by Dr. David A. D’Alessio 
  • Leptin and Diabetes by Dr. Jeffrey M. Friedman and Dr. Roger Unger
  • Beta Cell Regeneration: Novel Sources by Dr. Juan Domínguez-Bendala and Dr. Pedro Herrera
  • Advances in the Bionic Pancreas by Dr. Steven Russell
  • Glucagon Release with SGLT2 Inhibition by Dr. Ralph DeFronzo
  • Glucagon  - A Therapeutic Target - by Dr. Alan D Cherrington
  • Glucagon & Glicentin: Unique hormones from the Same Precursor - by Dr. Jens Juul Holst

ProSci's antibody protocols are available for a wide variety of assays including western blot, ELISA, immunofluorescence, immunohistochemistry, and immunocytochemistry.


Blog Articles

Molarity Calculator: Calculate the mass, volume or concentration required for a solution

Dilution Calculator: Calculate the dilution required to prepare a stock solution

Molecular Weight Calculator: Calculate the molar mass and elemental composition of a chemical using its chemical formula

Technical Resources